BIOTECHNOLOGY- PRINCIPLES AND PROCESSES
2013 MARCH
1. Jaya read in a
Biotechnology book that alien DNA can be introduced into host cell by
microinjection and biolistics. Explain these methods. (2)
2. Genetic
engineering includes creation of recombinant DNA with the help of restriction
enzymes.
a. Explain
recombinant DNA technology.
b. What are
restriction enzymes? Name a restriction enzyme. (2)
2013 SAY
1. Gel
electrophoresis is a technique to separate fragments of DNA from a mixture.
Some of the events of electrophoresis are given below. Arrange the events in
order: (3)
a. Cut out DNA
bands
b. Expose to UV
c. Force DNA to
move through gel
d. Stain DNA with
ethidium bromide
2. (b) Identify palindrome sequence from the
following. (1)
2014 MARCH
1. ------------
are the enzymes used for cutting the DNA molecule into fragments. An example
for this type of enzyme is Eco RI. What does Eco, R and I stand for? (2)
2. Use of a
thermostable DNA polymerase from the bacterium, Thermus aquaticus, made
it possible to generate billion copies of DNA in a very short time using a
process.
a. Name the
process (½)
b. Name the three
important steps involved in this process. (1½)
2014 SAY
1.
Consider you are appointed as biotechnologist in a
National Institute. What are the basic steps to be designed to produce a
genetically modified organism? (Hint: 3 points) (2)
2.
There are many features required to facilitate
successful cloning into a vector. Write shortly any two such features required
by a vector. (2)
2015 MARCH
1.
Figure representing the reactions associated with
Polymer Chain Reaction (PCR). Name the steps A, B, C in the process. (Score:
2)
2.
Recombinant DNA technology can be accomplished only
if we have the following key tools i.e. Restriction enzymes. Polymerase enzyme,
Ligases and Vectors.
State the
functions of (a) Ligases (b) Restriction
enzymes (Scores: 1 + 1
= 2)
2015 SAY
1.
Observe the cloning vector and explain the following:
a.
Ori b. BAM HI (Scores:
1+1 = 2)