BIOTECHNOLOGY- PRINCIPLES AND PROCESSES
2010 MARCH
1. Raju is searching blight resistant plants
for his crossing experiments. Unfortunately no such varieties are available.
a. Suggest a process to create such plants.
b. Justify your suggested method. (2)
2. Rinku with a circular DNA contains
sequence
5’ →
GGAATTCC → 3’
3’ →
CCTTAAGG → 5’
She wishes to add a new segment of DNA
into it.
a. Identify the technology she planned.
b. Suggest the specific enzyme to make a cut
in the DNA with above sequence.
c. Name the category of enzyme you
suggested.
d. How this enzyme identifies the sequence?
e. Draw the cut ends of the DNA with
sequence.
OR
Rashid
isolated a natural plasmid from a bacterium and planning to facilitate cloning.
a. What are the minimum requirements for
considering the isolated plasmid as a vector?
b. How he identifies whether a foreign DNA
is inserted or not after cloning? (4)
2010 SAY
1.
Diagram
shows a typical agarose gel showing migration of DNA fragments.
a. Which of the bands has largest and
smallest DNA fragments?
b. How can you make fragments of DNA for
electrophoresis?
c. Explain separation of DNA fragments using
electrophoresis.
d. Point out a method to visualize the
separated DNA fragments after electrophoresis. (4)
2011 MARCH
1.
The
picture given below shows the technique used for generating multiple copies of
the gene of interest.
a. What is the technique called?
b. Name the reactions at Step I, Step II,
Step III.
c. Explain the principle underlying this technique
of DNA amplification. (4)
OR
The above picture shows cloning vector
pBR322.
a. What is ori? Give its importance.
b. How does the insertion of foreign DNA at
Bam HI site selected? What is ampR?
c. How many cloning sites are depicted in
this vector as shown in the figure? (4)
2011 SAY
1. In a class room seminar on cloning
vectors, your friend asked to explain the steps to introduce the plasmid DNA to
a bacteria cell, Microinjection and bilestics. Answer his questions. (4)
2012 MARCH
1.
Restriction endonucleases are the enzymes used to
cut the DNA molecules.
a.
Give the general terms for the specific sequences
where these enzymes cut the DNA.
b.
Name the enzyme that joins the foreign DNA and
vector DNA.
c.
Give any two procedures to introduce the recombinant
DNA into the host cell. (4)
OR
During genetic
engineering Vector with foreign DNA is transferred into a host bacterium. The
next target will be the selection of transformants from non-transformants. How
antibiotic resistance and insertional inactivation is exploited for this
purpose? (4)
2012 SAY
1.
While
studying nucleotide sequence, Raj found that following sequence which can be
recognized by some enzymes: (4)
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
a. Give salient features of this sequence.
b. Write name of enzymes which recognize
such sequences.
c. Elaborate importance of this enzyme in
Genetic engineering.
OR
A group of students came to know about recombinant DNA technology.
They want to know how scientists can produce a new desired product using rDNA
technology. Can you give them an idea about the important steps that are
involved in this process? (4)