Plus 2 Biology Question Paper (PDF) with Answers | 2025 March

Plus Two Biology March 2025

SECOND YEAR HIGHER SECONDARY
EXAMINATION, MARCH 2025

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PART A: BOTANY

I. Answer any 3 questions from 1 to 5. Each carries 1 score. (3 x 1=3)

01. Decomposition would be fast if the detritus contains

(a) Cellulose

(b) Lignin and chitin

(c) Nitrogen and water soluble sugars

(d) Cellulose and pectin

✅ (c) Nitrogen and water soluble sugars
02. Name the method in which the alien DNA is directly injected into the nucleus of an animal cell.
✅ Micro-injection.
03. Observe the first pair and fill in the blanks.

Population interaction in which one species benefits and other is neither harmed nor benefited: Commensalism

Population interaction where one species is harmed and other is unaffected: ________

✅ Amensalism.
04. If any protein coding gene is expressed in a heterologous host, the protein formed is called ________.
✅ Recombinant protein.
05. Which among the following is a non-albuminous seed?

(Wheat, Groundnut, Maize, Barley)

✅ Groundnut.

II. Answer any 9 questions from 6 to 16. Each carries 2 scores. (9 X 2 = 18)

06. (a) Tapetum play an important role in the development of male gametophyte. Give reason.

(b) Write any one peculiarity of tapetal cell.

Answer:

(a) Tapetum nourishes the developing pollen grains (male gametophytes).

(b) Tapetal cells possess dense cytoplasm and generally multinucleate.

07. Write the steps in making the bacterial cell competent to take up the recombinant DNA.
Answer:

● Treating the bacterial cells with a specific concentration of a divalent cation, such as calcium.

● Incubating the cells with recombinant DNA on ice.

● Placing them briefly at 42°C (heat shock).

● Putting them back on ice.

08. (a) Define Gross primary productivity.

(b) What are the factors affecting primary productivity?

Answer:

(a) Gross primary productivity (GPP) is the rate of production of organic matter during photosynthesis in an ecosystem.

(b) Sunlight, temperature, moisture, photosynthetic capacity, plants in that area, availability of nutrients etc.

09. Apomixis is a blessing to agriculture. Justify the statement with reasons.
Answer:

Apomixis is the production of seeds without fertilization.

• It allows farmers to keep using hybrid seeds year after year because the desirable characters do not segregate in the progeny.

• It reduces the cost of seeds for farmers as they don't need to buy expensive hybrid seeds every season.

10. (a) What is population density?

(b) List any two ways of measuring population density of a habitat.

Answer:

(a) Population density refers to the number of individuals of a species per unit area or volume at a given time.

(b) (i) Percent cover or biomass.

(ii) Relative density (e.g., number of fish caught per trap). (iii) Indirect estimation (e.g., tiger census based on pug marks and faecal pellets).

11. Match the following:
1. ADA deficiencyAIDS
2. Human alpha-lactalbuminEmphysema
3. ELISAGene therapy
4. alpha-1 AntitrypsinRosie
Answer:
1. ADA deficiencyGene therapy
2. Human alpha-lactalbuminRosie
3. ELISAAIDS
4. alpha-1 AntitrypsinEmphysema
12. The most spectacular and evolutionarily fascinating examples of mutualism are found in plant-animal relationship. Elucidate with examples.
Answer:

Plants need animals for pollinating their flowers and dispersing their seeds, and animals are rewarded with pollen/nectar or juicy fruits.

Example 1: The Mediterranean orchid Ophrys employs 'sexual deceit' to get pollination done by a species of bee.

Example 2: Fig trees and wasps. The wasp pollinates the fig inflorescence while looking for egg-laying sites, and the fig provides developing seeds as food for the wasp larvae.

13. Give suitable terms for the following:

(a) A technique in plant biotechnology in which two different plant protoplasts are fused to create a hybrid with desirable traits from different varieties of plants.

(b) Genetically identical plants obtained by micropropagation.

Answer:

(a) Somatic hybridization.

(b) Somaclones.

14. The species facing competition might evolve mechanism that promote co-existence rather than exclusion. Evaluate the statement.
Answer:

This refers to Resource Partitioning. If two species compete for the same resource, they could avoid competition by choosing different times for feeding or different foraging patterns.

Example: MacArthur showed that five closely related species of warblers living on the same tree were able to avoid competition and co-exist due to behavioral differences in their foraging activities.

15. (a) Write the first trophic level of Grazing food chain and detritus food chain.

(b) In aquatic ecosystem, which food chain is the major conduit for energy flow?

Answer:

(a) Grazing Food Chain (GFC): Producers (Living plants).

Detritus Food Chain (DFC): Dead organic matter (Detritus).

(b) Grazing Food Chain (GFC).

16. Pathogens are used very effectively in biotechnology to transfer the DNA into host cells (plant and animal cell). Substantiate with suitable example.
Answer:

Pathogens are "disarmed" to serve as vectors.

Plant cells: Agrobacterium tumefaciens uses its Ti-plasmid to deliver genes into plants. The T-DNA is replaced with the gene of interest.

Animal cells: Retroviruses are disarmed and used to deliver desirable genes into animal cells.

III. Answer any 3 questions from 17 to 20. Each carries 3 scores. (3 X 3 = 9)

17. (a) What are the adaptations of water pollinated flowers?

(b) Give examples for the following:
(i) A water pollinated plant where pollen released on the surface of water
(ii) Water pollinated plant where pollen released inside the water

Answer:

(a) Adaptations: Pollen grains are protected from wetting by a mucilaginous covering. Flowers are often small and inconspicuous. They do not produce nectar or fragrance.

(b) (i) Vallisneria. (ii) Zostera (Sea grasses).

18. For effective treatment of a disease, early diagnosis and understanding its pathophysiology is very important. Early detection is possible through molecular diagnosis. Explain three molecular diagnostic tools for the early detection of disease.
Answer:

● Recombinant DNA technology: Allows for the detection of specific gene sequences.

● Polymerase Chain Reaction (PCR): Can detect pathogens (like HIV) even at very low concentrations by amplifying their nucleic acids.

● Enzyme Linked Immuno-sorbent Assay (ELISA): Based on the principle of antigen-antibody interaction to detect infections.

19. Given below the diagram of two bioreactors.

(a) Identify (i) and (ii).

(b) Compare the functioning mechanism of the two reactors.

Answer:

(a) (i) Simple stirred-tank bioreactor.

(ii) Sparged stirred-tank bioreactor.

(b) Simple stirred-tank: Has a stirrer that facilitates even mixing and oxygen availability throughout the bioreactor.

Sparged stirred-tank: Sterile air is bubbled through the reactor (sparging). These bubbles dramatically increase the surface area for oxygen transfer.

20. Given below the organisms of a Grassland eco-system.

Grasshopper, Grass, Snake, Frog

(a) Draw the pyramid of numbers.

(b) Pyramid of biomass in a marine eco-system is inverted. Give reason.

(c) Write any one limitation of ecological pyramids.

Answer:

(a) Pyramid of Numbers:

PICTURE

(b) This is because the biomass of fishes (consumers) far exceeds the biomass of phytoplankton (producers) at any given time.

(c) Limitations: It does not take into account the same species belonging to two or more trophic levels. It assumes a simple food chain and does not accommodate a food web. Saprophytes are not given any place.

PART B: ZOOLOGY

I. Answer any 3 questions from 1 to 5. Each carries 1 score. (3 x 1=3)

01. Name the oral contraceptive for female developed by CDRI.
Saheli.
02. Exaggerated response of the immune system to certain antigens present in the environment is called ________.
✅ Allergy.
03. Note the relationship between the first two words and fill the missing word.

(a) Primary sex organ in male: Testes

Primary sex organ in female: ________

(b) Leydig cells: Androgen

Corpus luteum: ________

✅ (a) Ovary. (b) Progesterone.
04. How many Thymine bases will a double stranded DNA have, if it has 30 Adenine bases?
✅ 30
05. Select the genetic disorder in which a blood clotting protein is affected leading to a non-stop bleeding even through a simple wound.

(Phenylketonuria, Haemophilia, Thalassemia, Sickle-cell Anaemia)

✅ Haemophilia.

II. Answer any 9 questions from 6 to 16. Each carries 2 scores. (9 X 2 = 18)

06. In a debate conducted in school, a student argued that, 'AIDS can be transmitted through touch or physical contact'.

(a) Do you agree with this statement? Substantiate.

(b) Name the clinical test used to diagnose AIDS.

(c) Write any one measure to prevent AIDS.

Answer:

(a) No, I do not agree. AIDS is not spread by mere touch or physical contact; it spreads only through body fluids (blood, semen, etc.).

(b) ELISA (Enzyme-Linked Immuno-Sorbent Assay).

(c) Use of disposable needles and syringes / Using condoms during intercourse.

07. Complete the table:
MicroorganismUses of Microorganism
____(a)____Curdling of milk
Bacillus thuringiensis____(b)____
Aspergillus niger____(c)____
____(d)____Production of butyric acid
Answer:

(a) Lactic Acid Bacteria (LAB).

(b) Biocontrol (agent).

(c) Citric Acid.

(d) Clostridium butylicum.

08. Observe the diagram showing lac operon in E. coli.

(a) Name the inducer.

(b) What do the letters 'o' and 'p' in lac operon stand for?

Answer: (a) Lactose (or allolactose). (b) 'o' stands for Operator; 'p' stands for Promoter.
09. Complete the flow-chart showing human evolution:

Dryopithecus → A → Australopithecines → B → C → Neanderthal Man → D

Answer:

A: Ramapithecus

B: Homo habilis

C: Homo erectus

D: Homo sapiens

10. Given below is a table showing codons of various amino acids. Observe it and write the name of:

(a) Stop codons

(b) Initiator codon

Answer:

(a) UAA, UAG, UGA.

(b) AUG.

11. Expand: (a) ZIFT (b) ICSI
Answer: (a) Zygote Intra Fallopian Transfer.

(b) Intra Cytoplasmic Sperm Injection.

12. Match the following:
Column AColumn B
Physical BarrierNeutrophil
Physiological BarrierSkin
Cellular BarrierInterferon
Cytokine BarrierSaliva in mouth
Answer:

Column AColumn B
Physical BarrierSkin
Physiological BarrierSaliva in mouth
Cellular BarrierNeutrophil
Cytokine BarrierInterferon
13. First child of a couple was affected with sickle-cell anaemia. During the second pregnancy, they visited a genetic counsellor and he prepared a pedigree chart of their family. Identify these symbols used in a pedigree chart.
Answer:

(a) Mating.

(b) Mating between relatives (Consangineous mating)

.

(c) Affected female.

(d) Unaffected male.

14. Observe the diagram and answer about oviduct.

(a) Projections for ovum collection.

(b) Part joins uterus.

(c) Part near ovary.

(d) Site of fertilisation.

Answer:

(a) Fimbriae.

(b) Isthmus.

(c) Infundibulum.

(d) Ampullary region.

15. Snapdragon plants have red flowers and white flowers. If red flowered plants are crossed with white flowered ones, F1 generation produce pink flowers.

(a) Name this pattern of inheritance.

(b) Write the genotypic and phenotypic ratio of F2.

Answer:

(a) Incomplete dominance.

(b) Phenotypic ratio: 1:2:1.

Genotypic ratio: 1:2:1.

16. In a population, the allele frequency of a species is p2+2pq+q2=1.

(a) Name the principle.

(b) Mention any 3 factors that may affect it.

Answer:

(a) Hardy-Weinberg Principle.

(b) Gene migration (Gene flow), Genetic drift, Mutation, Genetic recombination, Natural selection (Any three).

III. Answer any 3 questions from 17 to 20. Each carries 3 scores. (3 X 3 = 9)

17. (a) Identify pictures 'A' and 'B'.

Hint: A - IUD, B - Sterilisation procedure in male

(b) Amniocentesis for sex-determination is banned in our country. Is this ban necessary? Comment.

Answer:

(a) A: CuT (Copper T). B: Vasectomy.

(b) Yes, the ban is necessary to prevent female foeticide, which is essential to maintain a healthy sex ratio in society.

18. (a) Identify and write the name of the experiment shown in figure.

(b) What was the aim of this experiment?

(c) Name the processes marked as 'A', 'B' and 'C' (3 steps).

Answer:

(a) Hershey-Chase Experiment (Blender Experiment).

(b) To prove that DNA is the genetic material.

(c) A: Infection, B: Blending, C: Centrifugation.

19. The accelerated rates of species extinction that the world is facing now are largely due to human activities.

'Evil Quartet' is the sobriquet used to describe biodiversity loss.

(a) List out the 4 major causes of biodiversity loss.

(b) Mention 2 major approaches to biodiversity conservation.

Answer:

(a) 1. Habitat loss and fragmentation

      2. Over-exploitation

      3. Alien species invasions

      4. Co-extinctions.

(b) 1. In-situ conservation (e.g., National parks)

2. Ex-situ conservation (e.g., Zoological parks).

20. Male heterogamety is seen in humans and Drosophila.

(a) What is male heterogamety?

(b) How is sex determined in man?

(c) Is sperm or egg responsible for sex determination in chicks? Explain.

Answer:

(a) Male heterogamety is the production of two different types of gametes (sperms) by the male (e.g., 50% with X chromosome and 50% with Y chromosome).

(b) Sex is determined by the type of sperm fertilizing the egg. If a sperm with an X chromosome fertilizes the egg, the child is female (XX). If a sperm with a Y chromosome fertilizes the egg, the child is male (XY).

(c) Egg (Female heterogamety). In chicks, the female (ZW) produces two types of eggs, while the male (ZZ) is homogametic. Therefore, the egg determines the sex.

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